A2066
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 | Immunoblotting Chicken gizzard whole cell extracts were separated on SDS-PAGE, blotted with Rabbit Anti-Actin (Cat. No. A2066) and developed using Goat Anti-Rabbit IgG, Peroxidase (Cat. No. A0545) and AEC substrate. (Lane A) Antibody dilution 1:200; (Lane B) Negative control (only secondary antibody). |
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A2066
Sigma
Anti-Actin antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
| MDL number: | MFCD00145889 |
Description
| Frequently Asked Questions | Live Chat and Frequently Asked Questions are available for this Product. |
| Immunogen | The C-terminal actin fragment (C11 peptide, Ser-Gly-Pro-Ser-Ile-Val-His-Arg-Lys-Cys-Phe) attached to Multiple Antigen Peptide (MAP) backbone). |
| Other Notes | To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as preservative. |
| Application | May be used to study actin structure and function and to probe binding sites of actin-binding proteins. |
Properties
| antibody form | affinity isolated antibody |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt 42 kDa |
| species reactivity | amoeba, wide range, slime mold, vertebrates |
| application(s) | immunoblotting: 1:100 using human or animal tissue extracts |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using human or animal tissue sections following enzymatic unmasking | |
| immunohistochemistry (frozen sections): suitable using human or animal tissue sections following enzymatic unmasking | |
| indirect immunofluorescence: suitable using cultured chicken fibroblasts | |
| Gene Information | human ... ACTA1(58) |
| rat ... Acta1(29437) | |
| mouse ... Acta1(11459) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | NWG |
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References
| reference | Kayalar, C., et al., Cleavage of actin by interleukin 1 β-converting enzyme to reverse DNase I inhibition. Proc. Natl. Acad. Sci. U. S. A. 93, 2234, (1996) Abstract |
| Schneider, C., et al., The calpain system is involved in the constitutive regulation of beta-catenin signaling functions. J. Biol. Chem. 280, 22070-22080, (2005) Abstract | |
| Hata, K., et al., CD40 ligand rescues inhibitor of differentiation 3-mediated G1 arrest induced by anti-IgM in WEHI-231 B lymphoma cells. J. Immunol. 173, 2453-2461, (2004) Abstract | |
| Saunders, D.N., et al., Plasminogen binding and activation at the breast cancer cell surface: the integral role of urokinase activity Breast Cancer Res. 9, R14, (2007) Abstract |
