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Sigma
Anti-Actin antibody produced in rabbit
A2066 -
affinity isolated antibody, buffered aqueous solution
DOWNLOAD MSDS (PDF)MDL number: MFCD00145889
Properties
| Related Categories | AC-AC, Actin Antibodies, Alphabetical Index, Antibodies, Antibodies to Actin Isoforms, |
| antibody form | affinity isolated antibody |
| clone | polyclonal |
| form | buffered aqueous solution |
| mol wt | antigen mol wt 42 kDa |
| species reactivity | amoeba, wide range, slime mold, vertebrates |
| application(s) | immunoblotting: 1:100 using human or animal tissue extracts |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using human or animal tissue sections following enzymatic unmasking | |
| immunohistochemistry (frozen sections): suitable using human or animal tissue sections following enzymatic unmasking | |
| indirect immunofluorescence: suitable using cultured chicken fibroblasts | |
| shipped in | dry ice |
| storage temp. | −20°C |
| Gene Information | Research your gene in Your Favorite Gene powered by Ingenuity human ... ACTA1(58) mouse ... Acta1(11459) rat ... Acta1(29437) |
Description
Frequently Asked Questions
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Immunogen
The C-terminal actin fragment (C11 peptide, Ser-Gly-Pro-Ser-Ile-Val-H
Other Notes
To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/acti
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as preservative.
Application
May be used to study actin structure and function and to probe binding sites of actin-binding proteins.
Price and Availability
Antibody Bioguarantee
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody.
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affinity isolated antibody, buffered aqueous solution
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Documents
Certificate of Analysis
Certificate of Origin
| A2066 - Datasheet (53 KB) |
| A2066-IHC (14 KB ) |
| A2066-blot (12 KB ) |
| Gene Information |
| Antibody Explorer |  |
| Compatible Secondary Abs |  |
| Similar Products |
| Ask A Scientist |
Safety Information
| WGK Germany | - |
Technical information & documentation associated with this product is available in the Safety & Documentation tab.
References
Kayalar, C., et al., Cleavage of actin by interleukin 1 β-converting enzyme to reverse DNase I inhibition. Proc. Natl. Acad. Sci. U. S. A. 93, 2234, (1996) Abstract
Schneider, C., et al., The calpain system is involved in the constitutive regulation of beta-catenin signaling functions. J. Biol. Chem. 280, 22070-22080, (2005) Abstract
Hata, K., et al., CD40 ligand rescues inhibitor of differentiation 3-mediated G1 arrest induced by anti-IgM in WEHI-231 B lymphoma cells. J. Immunol. 173, 2453-2461, (2004) Abstract
Saunders, D.N., et al., Plasminogen binding and activation at the breast cancer cell surface: the integral role of urokinase activity Breast Cancer Res. 9, R14, (2007) Abstract
Block, K., et al., Nox4 NAD (P) H Oxidase Mediates Src-dependent Tyrosine Phosphorylation Of PDK-1 In Response To Angiotensin II J. Biol. Chem. 283, 24061-76, (2008) Abstract
Zhu, W., and Depamphilis, M.L., Selective Killing Of Cancer Cells By Suppression Of Geminin Activity. Cancer Res. 69, 4870-7, (2009) Abstract
Kuwahara, Y., et al., Reexpression Of HSNF5 In Malignant Rhabdoid Tumor Cell Lines Causes Cell Cycle Arrest Through A P21CIP1/WAF1-Dependent Mechanism Cancer Res. 70, 1854 - 1865, (2010) Abstract
Fujimoto, T., et al., Altered Energy Homeostasis And Resistance To Diet-induced Obesity In KRAP-deficient Mice. PLoS ONE 4, e4240, (2009) Abstract
Jiao, H., et al., Genetic Association And Gene Expression Analysis Identify FGFR1 As A New Susceptibility Gene For Human Obesity. J. Clin. Endocrinol. Metab. 96, E962-6, (2011) Abstract
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